Biochemical and Phosphoproteomic Analysis of the Helix-Loop-Helix Protein E47
نویسندگان
چکیده
منابع مشابه
Thymocyte Maturation Is Regulated by the Activity of the Helix-Loop-Helix Protein, E47
The E2A proteins, E12 and E47, are required for progression through multiple developmental pathways, including early B and T lymphopoiesis. Here, we provide in vitro and in vivo evidence demonstrating that E47 activity regulates double-positive thymocyte maturation. In the absence of E47 activity, positive selection of both major histocompatibility complex (MHC) class I- and class II-restricted...
متن کاملThe helix-loop-helix factors Id3 and E47 are novel regulators of adiponectin.
Adiponectin is an adipocyte-derived cytokine with beneficial effects on insulin sensitivity and the development of atherosclerosis. Id3 is a helix-loop-helix factor that binds to E-proteins such as E47 and inhibits their binding to DNA. Although the helix-loop-helix factor sterol regulatory element binding protein (SREBP)-1c is a known activator of adiponectin transcription, this study provides...
متن کاملRegulation of T cell receptor beta gene rearrangements and allelic exclusion by the helix-loop-helix protein, E47.
Allelic exclusion of antigen-receptor genes is ensured primarily by monoallelic locus activation upon rearrangement and subsequently by feedback inhibition of continued rearrangement. Here, we demonstrated that the basic helix-loop-helix protein, E47, promoted T cell receptor beta (TCRbeta) gene rearrangement by directly binding to target gene segments to increase chromatin accessibility in a d...
متن کاملMutations that disrupt DNA binding and dimer formation in the E47 helix-loop-helix protein map to distinct domains.
A common DNA binding and dimerization domain containing an apparent "helix-loop-helix" (HLH) structure was recognized recently in a number of regulatory proteins, including the E47 and E12 proteins that bind to the kappa E2 motif in immunoglobulin kappa gene enhancer. The effect of site-directed mutagenesis on E47 protein multimerization and DNA binding was examined. Mutations in either putativ...
متن کاملID helix-loop-helix proteins
The helix-loop-helix (HLH) family of transcription factors comprises >200 members, which have been identified in organisms from yeast to man (reviewed in Littlewood and Evan, 1995; Massari and Murre, 2000). In metazoa, HLH proteins function in the coordinate regulation of gene expression, orchestrating cell cycle control, cell lineage commitment and cell differentiation. An essential role has b...
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ژورنال
عنوان ژورنال: Molecular and Cellular Biology
سال: 2012
ISSN: 0270-7306,1098-5549
DOI: 10.1128/mcb.06452-11